What is NAD+?
Nicotinamide adenine dinucleotide (NAD⁺) is a vital coenzyme present in every living cell. It plays a central role in cellular energy production, DNA repair, and metabolic regulation. NAD⁺ levels decline with age, a change linked to reduced mitochondrial function and increased cellular stress. Buffered NAD⁺ formulations are designed to improve tolerability and stability during supplementation or intravenous administration.
Why is NAD+ Important?
NAD⁺ is essential for converting nutrients into usable energy. It also serves as a substrate for enzymes involved in DNA repair (PARPs) and longevity pathways (sirtuins). Adequate NAD⁺ levels support metabolic efficiency and cellular resilience.
How Does NAD+ Work?
NAD+ is an energy-carrying molecule that is used as a source of energy for biologic processes, especially those involving oxidation/reduction reactions. NAD+ can be thought of as similar to ATP, which is the more widely-known energy-carrying molecule. ATP acts as a more immediate source of energy, like gasoline for a car. NAD+ is more indirect energy, like the electrical system that enables the engine to run efficiently.
What is NAD+ Used for?
In the research realm, NAD+ can potentially help in the following avenues:
- Age-related metabolic decline
- Neuroprotection and cognitive health
- Cellular energy and fatigue
- DNA repair and stress resistance
Side Effects of NAD+
In research uses, NAD+ is reported to be rarely associated with flushing or nausea if infused too rapidly. Long-term safety data is still evolving.
NAD+ Dosing Protocol (for Research)
NAD+ research protocols call for twice-weekly administration under the skin of a research subject (subcutaneous).
| Phase | Dose & frequency |
|---|---|
| Dosing protocol | 50 mg twice weekly (no up-titration needed) |
Shown for education. A licensed clinician should set any actual dose. Compare protocols across peptides in the peptide dosage chart.
Common questions about NAD⁺ (buffered)
What is NAD+ (buffered)?
Nicotinamide adenine dinucleotide (NAD⁺) is a vital coenzyme present in every living cell. It plays a central role in cellular energy production, DNA repair, and metabolic regulation. NAD⁺ levels decline with age, a change linked to reduced mitochondrial function and increased cellular stress.
What is NAD+ (buffered) used for?
Areas studied include: Age-related metabolic decline; Neuroprotection and cognitive health; Cellular energy and fatigue; DNA repair and stress resistance.
How does NAD+ (buffered) work?
NAD+ is an energy-carrying molecule that is used as a source of energy for biologic processes, especially those involving oxidation/reduction reactions. NAD+ can be thought of as similar to ATP, which is the more widely-known energy-carrying molecule.
What are the side effects of NAD+ (buffered)?
In research uses, NAD+ is reported to be rarely associated with flushing or nausea if infused too rapidly. Long-term safety data is still evolving.
What is the dosage of NAD+ (buffered)?
One commonly referenced research protocol is: 50 mg twice weekly (no up-titration needed). Shown for education only; a licensed clinician should set any actual dose. See the dosing table.
How do you mix (reconstitute) NAD+ (buffered)?
Wipe the vial tops with alcohol, add bacteriostatic water slowly so it runs down the inside of the glass, then roll the vial gently until the powder dissolves; don’t shake it. See the step-by-step guide with diagrams for the pen and syringe methods.
How much bacteriostatic water do you add to NAD+ (buffered)?
There is no single correct amount. We recommend 2.1 mL: about 0.1 mL is lost during transfer, leaving roughly 2.0 mL of usable solution. You can add more or less (most peptide vials hold up to 3 mL); just enter the amount in the dose calculator. See the step-by-step mixing guide.
Is NAD+ (buffered) FDA-approved?
No. NAD⁺ (buffered) is not FDA-approved in the US (status as of October 2026).
References
- Verdin E. NAD⁺ in aging, metabolism, and neurodegeneration. Science. 2015;350(6265):1208-13. doi: 10.1126/science.aac4854. View source
- Yoshino J, et al. NAD+ Intermediates: The biology and therapeutic potential of NMN and NR. Cell Metab. 2018 Mar 6;27(3):513-28. doi: 10.1016/j.cmet.2017.11.002. View source
- Rajman L, et al. Therapeutic potential of NAD-boosting molecules: the in vivo evidence. Cell Metab. 2018;27:529-47. doi: 10.1016/j.cmet.2018.02.011. View source